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. 2013 May 9;288(27):20014–20033. doi: 10.1074/jbc.M112.438895

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Exosomes isolated from HIV-1-infected cells possess typical characteristics. A, Jurkat and J1.1-derived exosomes obtained from 5-day-old cell culture supernatants and corresponding WCEs were resolved in a Tris-glycine gel and silver-stained. The band migrating close to 65 kDa was excised from both the Jurkat and J1.1-exosome lanes and analyzed by MALDI-TOF MS. B, Jurkat and J1.1-derived exosomes and corresponding WCEs were analyzed by Western blots with antibodies to CD63, CD45, Hsp70, Alix, cytochrome c, and β-actin. C, Jurkat and J1.1-derived exosomes were observed by transmission electron microscopy and the multiple representative exosomes indicated.