Figure 2.

The degree of the tumor population is dependent on the number of retinoblastoma cells injected. We injected 20 and 100 retinoblastoma cells into the vitreous cavity of zebrafish, and the eyeballs of zebrafish embryos were scanned daily through the confocal laser microscope 20 cells, (A; 100 cells, B). Scale bar, 100 μm. The tumor population was quantitatively analyzed as the mean intensity of GFP expression at 4 dpi with the public image processing program, ImageJ (C). Data of 8 zebrafish per group from at least 2 independent experiments. dpi, day(s) post injection; GFP, green fluorescent protein.