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Up-regulation of Frizzled-8 and Wnt-2 in lung cancer. Semi-quantitative RT-PCR analysis of 50 freshly resected tumor samples with their corresponding matched, normal lung controls. Total RNA was reverse transcribed and amplified with primers specific for Wnt-2 or Frizzled-8. The data shown are representative tumor pairs (tumor (T) and normal (N)). Semi-quantitative RT-PCR products were resolved on a 1.5% agarose gel. Experiments were performed in triplicate.
