Figure 3.

Dominant negative human Wnt-2 inhibits Frizzled-8 activation. (A) Inhibition of TCF transcriptional activity by dnhWnt-2 construct was measured in 293T, 293 and A549 cells. In the present or absence of dnhWnt-2 construct, the cells were co-transfected with both Frizzled-8 and Wnt-2 expression vectors, either pTOPflash or pFOPflash, and internal control plasmid pRL-TK. Experiments were performed in triplicate. (B) Stabilization of β-catenin by dnhWnt-2 expression in the indicated cell lines was determined by measuring cytosolic β-catenin protein levels in the cells.