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. Author manuscript; available in PMC: 2013 Jul 10.
Published in final edited form as: Mol Cancer Res. 2012 Feb 1;10(4):535–545. doi: 10.1158/1541-7786.MCR-11-0508

Figure 2. Immunohistochemical profiling of A549 NSCLC xenografts indicates increased DNA damage and decreased proliferation following WRN knock-down.

Figure 2

A. Representative IHC images from A549 xenografts demonstrating decreased proliferation (H&E and Ki67), and increased DNA damage (γ-H2A.X, Serine139) in WRN depleted xenografts (shWRN I) vs. control xenografts (shCTL). In contrast no difference in apoptosis as judged by cleaved caspase-3 was detectable. H&E and Ki67 images show 10X field with 40X field inset. B. Quantitative scoring of DNA damage (H2A.X Serine 139) from control and shWRN xenografts. Bars represent mean+/−SD; n=3 mice per graft, ten 40x fields/mouse, ***p<0.0001 (unpaired t-test). C. Quantitative scoring of apoptosis (cleaved caspase-3) from control and shWRN xenografts. Bars represent mean+/−SD; n=3 mice per graft, ten 40x fields/mouse, no significant difference via unpaired t-test.