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The time dependency of the anxiolytic effect of an alkaline fraction from Biebersteinia multifida roots on mice. The elevated plus-maze test was performed 30 min and 90 min after injection. The animals were injected 30 min prior to the test with saline (vehicle), diazepam (1 mg/kg) or different doses of alkaline fraction. The ratio of percent time spent in open arms to close arms was calculated and plotted as mean ± SE for each group (n = 12 in each group).
