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. 2013 Jul 10;8(7):e68778. doi: 10.1371/journal.pone.0068778

Figure 3. Appearance and body weight of mice.

Figure 3

(A) Image of one 74 weeks ad libitum (AL) and one caloric-restricted (CR) fed C57BL/6J mouse. These mice exemplarily showed that in general CR mice were smaller in body size and had fewer external age-related signs as showing denser coat and barely greyed hair than the AL mice. (B) Starting weight (n4w, 20w, 74w = 20, 20, 17) and body weight (g) of the 4 (4w), 20 (20w) and 74 (74w) weeks ad libitum (AL) and caloric-restricted fed mice were shown (AL: n4w, 20w, 74w = 10, 10, 7; CR: n4w, 20w, 74w = 10, 10, 10). In all AL fed mice, 20 and 74 weeks CR fed mice body weight was significantly increased when compared to their starting weight (4 weeks: F1,28 = 197.69, P<0.001; 20 weeks: F1,28 = 364.85, P<0.001; 74 weeks: F1,22 = 346.88, P<0.001). Compared with the age-matched AL fed mice the CR fed mice had significantly lower weights (4 weeks: F1,18 = 176.14, P<0.001; 20 weeks: F1,18 = 442.02, P<0.001; 74 weeks: F1,15 = 156.76, P<0.001). Values are given as mean±SEM; ANOVA, post-hoc pairwise comparison tests: &P<0.05 vs. starting weight; §P<0.001 vs. AL.