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. 2013 Jul 10;8(7):e68778. doi: 10.1371/journal.pone.0068778

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© 2013 Kuhla et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–C) The escape latencies (in ms) of the training were displayed in five blocks (x-axis 1–5). One block consisted of four consecutive trials. The escape latencies decreased throughout the training days. (A–B) For the 4 (4w) and 20 weeks (20w) AL and CR fed groups two-way ANOVA for repeated measures (group vs. block) revealed no significance for the main factor group (4 weeks: F_1,17 = 0.934, P = 0.347; 20 weeks: F_1,18 = 1.523, P = 0.233), but a significant effect for the main factor block (F_1,4 = 29.753, P<0.001; F_1,4 = 62.468, P<0.001). The interaction between these variables is significant between 4 weeks AL and CR fed mice (F_1,4 = 4.807, P = 0.002), but no significance could be shown for interaction of 20 weeks fed groups (F_1,4 = 0.918, P = 0.458). Two-way ANOVA for repeated measures (group vs. block) of the 74 weeks (74w) AL and CR fed mice indicated a significant effect for the main factors (group: F_1,15 = 4.816, P = 0.044; block: F_1,4 = 25.078, P<0.001), but there was no statistically significant interaction between group and block (F_1,4 = 2.312, P = 0.068). Subsequent Holm-Sidak tests with an overall significance level of P = 0.05 showed only significant differences of escape latency in block 2 respectively between 4 and 20 weeks AL and CR fed mice (Fig. 6A–B). (C) Between 74 weeks AL and CR fed groups there were significant differences of escape latency in blocks 4 and 5. Values are given as mean±SEM; ANOVA, post-hoc pairwise comparison tests: ^§P<0.05 vs. AL. (D–F In this test trial, the number of platform crossings during 60 s was measured. (D and E) Two-way ANOVA of the 4 and 20 weeks fed groups (group vs. platform) showed no significances for the main factor group and for interaction between group and platform (4 weeks: group: F_1,72 = 0.536, P = 0.466; interaction: F_1,3 = 2.249, P = 0.090; 20 weeks: group: F_1,72 = 1.188, P = 0.279; interaction: F_1,3 = 0.644, P = 0.589). Values are given as mean±SEM; ANOVA, post-hoc pairwise comparison tests: ^#P<0.05 vs. PF4; ^§P<0.05 vs. AL. (F) The 74 weeks AL fed mice (n = 7) did not show significantly more crossings of PF4. Values are given as mean±SEM; ANOVA, post-hoc pairwise comparison tests: ^#P<0.05 vs. PF4; ^§P<0.05 vs. AL.