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. 2013 Jul 10;8(7):e70190. doi: 10.1371/journal.pone.0070190

Figure 3. Antigen binding capacity of monovalent IgGs displayed by Fc-Sed1p measured by flow cytometry.

Figure 3

The cells were dually labeled with goat anti-human Fc DyeLight 488, biotinylated PCSK9, and APC 635 labeled Streptavidin A) FACS analysis of labeled Pichia pastoris strains displaying Fc-Sed1p complexed with monovalent anti-Her2 antibody fragment (H+L) or B) an monovalent anti-PCSK9 (H+L) antibody fragment.