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. 2013 Jul 10;8(7):e68514. doi: 10.1371/journal.pone.0068514

Table 1. Summary of genetic diversity within 60 populations of Pinus strobus based on ten microsatellite loci.

Pop. Locality Latitude Longitude N A H S R S H O H E f(FIS)
E1 Doubice, Hrad N50°53′26″ E14°24′58″ 20 5.2 0.597 4.467 0.479 0.579 0.198*
E2 Doubice, Dravčí Skály N50°54′25″ E14°24′25″ 20 4.9 0.543 4.005 0.441 0.525 0.188*
E3 Hradec Králové N50°10′13″ E15°52′34″ 20 5.1 0.547 4.180 0.444 0.529 0.188*
E4 Klokočí N50°36′20″ E15°12′46″ 20 4.5 0.550 3.845 0.433 0.533 0.214*
E5 Brandýs-Stará Boleslav N50°13′02″ E14°44′05″ 20 5.5 0.569 4.478 0.433 0.550 0.240*
E6 Řečany nad Labem N50°01′38″ E15°28′59″ 20 5.2 0.614 4.362 0.500 0.594 0.185*
E7 Bílichov N50°14′38″ E13°52′08″ 21 5.2 0.543 4.302 0.372 0.524 0.314*
E8 Říčany N49°59′10″ E14°42′25″ 20 5.0 0.520 4.118 0.418 0.503 0.195*
E9 Jemčina N49°06′30″ E14°50′04″ 20 4.9 0.533 4.113 0.394 0.512 0.261*
E10 Adršpach N50°36′08″ E16°07′33″ 20 4.1 0.465 3.473 0.363 0.449 0.219*
E11 Studené N50°04′20″ E16°35′19″ 20 4.5 0.524 3.841 0.377 0.505 0.280*
E12 Blatce N50°30′53″ E14°36′05″ 19 5.0 0.544 4.274 0.459 0.526 0.156*
E13 Pařezská Lhota N50°28′28″ E15°16′43″ 20 6.0 0.517 4.689 0.385 0.499 0.255*
E14 Příhrazy N50°31′35″ E15°03′54 20 5.0 0.546 4.175 0.437 0.528 0.198*
E15 Aš N50°15′23″ E12°12′25″ 20 5.4 0.585 4.443 0.452 0.565 0.227*
E16 Kynšperk nad Ohří N50°08′32″ E12°31′42″ 21 4.7 0.586 4.042 0.441 0.568 0.248*
E17 Cvikov N50°46′31″ E14°36′02″ 20 5.4 0.545 4.482 0.433 0.528 0.206*
E18 Liberec N50°48′04″ E14°59′51″ 21 5.7 0.563 4.523 0.467 0.547 0.171*
E19 Bezděz N50°30′44″ E14°41′46″ 20 5.2 0.570 4.315 0.489 0.551 0.141*
E20 Petrovice N50°48′58″ E14°46′34″ 20 5.4 0.573 4.338 0.473 0.555 0.174*
E21 Česká Kamenice N50°48′36″ E14°27′35″ 20 4.7 0.479 3.793 0.367 0.463 0.234*
E22 Stráž pod Ralskem, Ralsko N50°40′53″ E14°51′36″ 20 5.2 0.585 4.500 0.455 0.565 0.221*
E23 Jablonec nad Nisou N50°43′06″ E15°07′03″ 20 5.8 0.575 4.546 0.396 0.555 0.312*
E24 Dvůr Králové nad Labem N50°24′45″ E15°47′50″ 20 5.2 0.526 4.131 0.400 0.509 0.241*
E25 Zbiroh N49°50′03″ E13°48′45″ 20 5.1 0.560 4.075 0.472 0.544 0.157*
E26 Plzeň N49°47′43″ E13°22′37″ 20 5.3 0.596 4.443 0.482 0.577 0.191*
E27 Horní Štěpánov N49°32′37″ E16°46′12″ 20 4.8 0.557 4.070 0.399 0.537 0.284*
E28 Huntířov, Bynovec N50°48′01″ E14°18′08″ 20 5.1 0.560 4.121 0.455 0.543 0.187*
E29 Srbská Kamenice N50°50′01″ E14°22′08″ 20 5.6 0.527 4.589 0.446 0.511 0.154*
E30 Sopřeč N50°04′26″ E15°32′54″ 20 4.8 0.547 4.075 0.429 0.528 0.216*
A31 NY, Millbrook N41°47′38″ W73°43′59″ 20 5.0 0.591 4.145 0.461 0.572 0.221*
A32 NY, New Paltz N41°45′42″ W74°09′46″ 20 5.2 0.476 4.097 0.422 0.462 0.114*
A33 NY, Albany N42°35′27″ W74°00′28″ 20 5.3 0.546 4.378 0.368 0.526 0.326*
A34 PA, Wellsboro N41°41′48″ W77°27′18″ 20 5.5 0.552 4.381 0.422 0.534 0.236*
A35 PA, Clarington N41°20′27″ W79°08′00″ 20 5.6 0.522 4.409 0.352 0.504 0.326*
A36 PA, Warren N41°42′04″ W79°14′56″ 20 4.4 0.516 3.782 0.433 0.497 0.161*
A37 NY, Castile N42°36′28″ W78°01′30″ 20 5.2 0.587 4.386 0.442 0.568 0.246*
A38 NY, Bergen N43°05′24″ W78°02′00″ 20 5.3 0.574 4.335 0.507 0.557 0.117*
A39 NY, Pulaski N43°33′43″ W76°11′40″ 20 5.9 0.526 4.515 0.400 0.509 0.240*
A40 NY, Tupper Lake N44°18′41″ W74°43′16″ 20 5.9 0.516 4.478 0.411 0.499 0.203*
A41 NY, Wilmington N44°21′46″ W73°50′32″ 20 5.7 0.559 4.575 0.468 0.540 0.162*
A42 VT, Johnson N44°39′11″ W72°43′38″ 20 5.8 0.577 4.673 0.417 0.557 0.277*
A43 NH, Bartlett N44°04′30″ W71°18′17″ 20 5.4 0.499 4.184 0.444 0.485 0.111*
A44 ME, Freeport N43°49′22″ W70°05′36″ 20 5.6 0.537 4.316 0.396 0.519 0.262*
A45 ME, Guilford N45°15′53″ W69°16′55″ 20 5.4 0.560 4.422 0.405 0.542 0.277*
A46 ME, Millinocket N45°35′47″ W68°48′51″ 20 5.2 0.548 4.154 0.467 0.532 0.149*
A47 ME, Seal Cove N44°16′40″ W68°22′45″ 20 4.1 0.484 3.376 0.468 0.471 0.033
A48 ME, New Harbor N43°54′48″ W69°29′12″ 20 5.5 0.567 4.459 0.461 0.548 0.187*
A49 MA, Chelmsford N42°33′31″ W71°20′55″ 20 5.3 0.536 4.359 0.473 0.521 0.117*
A50 MA, Taunton N41°58′42″ W71°04′52″ 20 5.5 0.548 4.311 0.466 0.532 0.150*
A51 VT, Manchester N43°07′41″ W73°07′05″ 20 5.9 0.559 4.659 0.487 0.543 0.128*
A52 MA, Shelburne Falls N42°37′30″ W72°46′13″ 20 5.8 0.551 4.653 0.453 0.533 0.179*
A53 CT, Mansfield Center N41°45′60″ W72°10′47″ 20 6.3 0.600 4.898 0.478 0.582 0.203*
A54 CT, Burlington N41°46′14″ W72°57′13″ 20 5.0 0.497 4.070 0.443 0.483 0.109*
A55 MI, Alger N44°04′04″ W84°10′58″ 20 5.2 0.559 4.306 0.489 0.543 0.125*
A56 MI, Pellston N45°32′54″ W84°41′58″ 20 5.2 0.602 4.458 0.433 0.581 0.280*
A57 MI, White Pine N46°44′89″ W89°42′48″ 20 6.2 0.592 4.843 0.453 0.572 0.235*
A58 MI, Marquette N46°37′21″ W87°28′20″ 20 5.5 0.56 4.490 0.486 0.543 0.132*
A59 MI, Sault Ste. Marie N46°21′23″ W84°08′22″ 20 6.1 0.536 4.884 0.432 0.518 0.195*
A60 MI, Bridgman N41°54′17″ W86°36′25″ 12 4.6 0.501 4.302 0.367 0.475 0.268*
Mean 5.3 0.549 4.302 0.437 0.531 0.204
SD 0.5 0.033 0.300 0.038 0.032 0.061

Pop. = Population – the first letter of each population name refers to the continent in which the population was found, i.e. E = Europe (Czech Republic) and A = North America; populations in bold were classified as invasive within the adventive range; GPS coordinates of sampling sites are in WGS84; N – number of individuals sampled from each population; A – average number of alleles per locus; H S – mean gene diversity over all loci; R S – mean allelic richness; H O – observed heterozygosity; H E – expected heterozygosity; f(FIS) – inbreeding coefficient according to Weir and Cockerham [77]. Populations deviating from Hardy-Weinberg equilibrium at P<0.05 are marked by asterisk. SD – standard deviation. The fine scale studies were performed at localities E1, E22, E28 and E30.