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. 2013 Jul 10;8(7):e66710. doi: 10.1371/journal.pone.0066710

Figure 4. TdIF1 promotes transcription in a luciferase reporter assay.

Figure 4

(A) TdIF1 promotes transcription. The pGL3-promoter-TdIF1-binding sequence and pRL-TK were co-transfected with pEGFP or pEGFP-TdIF1 into 293T cells, and the luciferase activity was assayed. The relative luciferase activity was normalized to the value obtained with the pGL3-promoter and pEGFP. Error bars represent S.E.M. Samples significantly different from the control are indicated by asterisks (p<0.01). (B) TdIF1 promotes transcription by directly binding to DNA. The pGL3-promoter-TdIF1-binding sequence and pRL-TK were co-transfected with pEGFP-TdIF1 truncated and point mutants, and the luciferase activity was assayed. Error bars represent S.E.M.