Table 1. Proteins identified from the differential 2D DIGE analysis (pH 3–10) after WNV infection.
| Accession number (SwissProt) | Protein name | Molecular weight (kDa) | pI | Spot ID | Number of MS/MS peptide sequences | Sequence coverage (%) | Mascot score | Average volume ratio | Anova (p-value) | ||
| WNV-E vs. mock | WNV-L vs. mock | WNV-L vs. WNV-E | |||||||||
| Viral proteins | |||||||||||
| POLG_KUNJM | Genome polyprotein [Kunjin virus] | 384.717 | 8.70 | 577 | 11 | 3.1 | 324 | 5.5 | 6.0 | 2.7e-8 | |
| 601 | 8 | 2.7 | 333 | 1.4 | 1.3 | 5.4e-5 | |||||
| 602 | 6 | 2.0 | 200 | 2.9 | 3.0 | 6.9e-6 | |||||
| 604 | 6 | 2.0 | 231 | 3.4 | 3.2 | 1.6e-6 | |||||
| POLG_WNV | Genome polyprotein [West Nile virus] | 383.401 | 8.63 | 710 | 1 | 0.3 | 77 | 1.4 | 0.003 | ||
| 779 | 1 | 0.3 | 98 | 6.4 | 5.4 | 4.3e-11 | |||||
| Host proteins | |||||||||||
| Metabolic/biosynthertic process | |||||||||||
| ACLY_MOUSE | ATP-citrate synthase [Mus musculus] | 120.564 | 7.13 | 325 | 9 | 10.0 | 231 | −1.4 | 0.005 | ||
| BACH_MOUSE | Cytosolic acyl coenzyme A thioester hydrolase [Mus musculus] | 42.966 | 8.90 | 903 | 3 | 8.7 | 70 | −1.3 | 8.7e-7 | ||
| CAH2_MOUSE | Carbonic anhydrase 2 [Mus musculus] | 29.129 | 6.49 | 1065 | 1 | 3.5 | 35 | 1.3 | 0.003 | ||
| CATA_MOUSE | Catalase [Mus musculus] | 60.403 | 7.72 | 701 | 8 | 16.9 | 205 | −1.3 | −1.3 | 1.5e-6 | |
| GPDM_MOUSE | Glycerol-3-phosphate dehydrogenase, mitochondrial [Mus musculus] | 81.416 | 6.17 | 1032 | 2 | 4.5 | 100 | 1.5 | 1.4 | 0.004 | |
| SYG_MOUSE | Glycyl-tRNA synthetase [Mus musculus] | 82.624 | 6.24 | 560 | 5 | 6.4 | 105 | −1.3 | −1.3 | 2.1e-5 | |
| Host response/protein folding | |||||||||||
| ALBU_MOUSE | Serum albumin [Mus musculus] | 70.700 | 5.75 | 578 | 22 | 35.0 | 1468 | −1.4 | 1.8 | 2.4 | 2.7e-7 |
| 580 | 14 | 25.8 | 1535 | −1.3 | 1.6 | 2.1 | 5.5e-7 | ||||
| 585 | 12 | 20.2 | 190 | 1.4 | 1.5 | 7.0e-6 | |||||
| 587 | 1 | 2.1 | 69 | 1.6 | 1.8 | 5.1e-7 | |||||
| 606 | 2 | 3.0 | 38 | 1.4 | 1.4 | 2.0e-6 | |||||
| 609 | 1 | 2.5 | 116 | 2.0 | 2.0 | 2.8e-7 | |||||
| HS12A_MOUSE | Heat shock 70 kDa protein 12A [Mus musculus] | 75.167 | 6.32 | 558 | 4 | 7.6 | 103 | −1.4 | −1.4 | 4.1e-5 | |
| HSP7C_MOUSE | Heat shock cognate 71 kDa protein [Mus musculus] | 71.055 | 5.37 | 578 | 10 | 19.0 | 231 | −1.4 | 1.8 | 2.4 | 2.7e-7 |
| 580 | 3 | 6.8 | 120 | −1.3 | 1.6 | 2.1 | 5.5e-7 | ||||
| 585 | 11 | 21.1 | 273 | 1.4 | 1.5 | 7.0e-6 | |||||
| 587 | 1 | 2.3 | 40 | 1.6 | 1.8 | 5.1e-7 | |||||
| 609 | 1 | 1.9 | 76 | 2.0 | 2.0 | 2.8e-7 | |||||
| PRDX6_MOUSE | Peroxiredoxin-6 [Mus musculus] | 24.969 | 5.71 | 1102 | 6 | 30.4 | 106 | 1.3 | 1.3 | 0.001 | |
| TCPA_MOUSE | T-complex protein 1 subunit alpha [Mus musculus] | 60.867 | 5.82 | 657 | 4 | 8.1 | 187 | −1.3 | −1.3 | 0.024 | |
| TCPG_MOUSE | T-complex protein 1 subunit gamma [Mus musculus] | 61.162 | 6.28 | 476 | 6 | 20.2 | 164 | 1.3 | 1.6 | 6.8e-5 | |
| Transcription/translation regulation | |||||||||||
| EF2_MOUSE | Elongation factor 2 [Mus musculus] | 96.222 | 6.41 | 380 | 21 | 25.3 | 609 | −1.3 | 0.006 | ||
| FUBP2_MOUSE | Far upstream element-binding protein 2 [Mus musculus] | 77.184 | 6.90 | 505 | 9 | 15.0 | 191 | 1.4 | 1.4 | 2.2e-5 | |
| SFPQ_MOUSE | Splicing factor, proline- and glutamine-rich [Mus musculus] | 75.508 | 9.45 | 386 | 3 | 3.4 | 46 | −2.0 | −1.6 | 1.2e-4 | |
| Cytoskeleton maintenanvce | |||||||||||
| ACTB_MOUSE | Actin, cytoplasmic 1 [Mus musculus] | 42.052 | 5.29 | 876 | 3 | 7.7 | 48 | 1.3 | 1.8 | 1.4 | 2.9e-8 |
| 882 | 8 | 25.9 | 241 | 1.6 | 1.4 | 2.1e-4 | |||||
| 1056 | 3 | 7.7 | 58 | 1.4 | 9.1e-4 | ||||||
| DYN1_MOUSE | Dynamin-1 [Mus musculus] | 98.140 | 7.61 | 475 | 9 | 13.0 | 170 | 1.3 | 1.6 | 1.3 | 3.6e-5 |
| 476 | 5 | 15.6 | 163 | 1.3 | 1.6 | 6.8e-5 | |||||
| TBB5_MOUSE | Tubulin beta-5 chain [Mus musculus] | 50.095 | 4.78 | 710 | 6 | 15.0 | 225 | 1.4 | 0.003 | ||
| Nervous system developement | |||||||||||
| DPYL2_MOUSE | Dihydropyrimidinase-related protein 2 [Mus musculus] | 62.638 | 5.95 | 558 | 9 | 20.1 | 510 | −1.4 | −1.4 | 4.1e-5 | |
| 560 | 9 | 20.1 | 298 | −1.3 | −1.3 | 2.1e-5 | |||||
| 625 | 12 | 26.7 | 739 | −1.3 | 2.0e-6 | ||||||
| DPYL3_MOUSE | Dihydropyrimidinase-related protein 3 [Mus musculus] | 62.296 | 6.04 | 657 | 13 | 29.1 | 989 | −1.3 | −1.3 | 0.024 | |
| GFAP_MOUSE | Glial fibrillary acidic protein [Mus musculus] | 49.927 | 5.27 | 792 | 16 | 34.5 | 385 | 1.4 | 1.3 | 2.2e-5 | |
| Transport | |||||||||||
| TRFE_MOUSE | Serotransferrin [Mus musculus] | 78.841 | 6.94 | 497 | 12 | 17.9 | 467 | 1.5 | 1.9 | 1.5e-7 | |
| 499 | 16 | 25.4 | 616 | 1.6 | 2.0 | 5.4e-7 | |||||
| 502 | 12 | 22.8 | 428 | 1.5 | 1.6 | 4.5e-6 | |||||
| VATB2_MOUSE | V-type proton ATPase subunit B, brain isoform [Mus musculus] | 56.857 | 5.57 | 710 | 2 | 4.1 | 70 | 1.4 | 0.003 | ||
| Not identified | |||||||||||
| n.i | 350 | −1.4 | −1.3 | 8.9e-5 | |||||||
| n.i | 481 | 1.4 | 2.4 | 1.8 | 1.1e-9 | ||||||
| n.i | 1132 | 1.3 | 0.002 | ||||||||
The proteins were identified by mass spectrometry following in-gel trypsin digestion. The spot numbers correspond to the same numbers as indicated on Figure 2. The identities of the spots, their SwissProt accession numbers, and the theoretical molecular masses and pI values as well as the number of peptide sequences, the corresponding percent sequence coverage, and the Mascot score are listed for MS/MS analysis. Protein scores greater than 34 were considered as significant (p<0.05). Paired average volume ratio and p values (ANOVA) between the three experimental groups were defined using Progenesis Samespot software. Missing data correspond to paired average volume ratio not significantly changes. n.i., no identification.