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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2013 Apr 25;12(7):1180–1189. doi: 10.1158/1535-7163.MCT-12-1176-T

Figure 4. SEN461 affects canonical WNT ligands mediated transcription and modulates molecular markers of the pathway.

Figure 4

HEK293 cells, transiently transfected with TCF-Luciferase and TA-Renilla and different combination of WNT1 (A) and WNT3A (B) expression plasmids were either treated with DMSO (vehicle) or different amounts of SEN461. The data showed potent concentration-dependent inhibition of WNT transcriptional activity either in WNT1 or WNT3A mediated luciferase activity, without affecting WNT-independent TA-Renilla activity. Data represent means ± SEMs. HEK293 cells were stimulated overnight with Wnt3a conditioned medium or control conditioned medium, alone or in combination with SEN461. Lysates were analyzed by Western blotting with total and phosphorylated anti-β-CATENIN and anti-AXIN1 (C). The effect of SEN461 treatment after Wnt3a CM stimulation on the WNT target gene AXIN2 (mRNA) was measured by quantitative RT-PCR (D). Data represent means ± SEM. *P<0.001 relative to Wnt3a stimulated cells (Tukey’s Multiple Comparison Test).