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. 2013 Jul 10;8(7):e68753. doi: 10.1371/journal.pone.0068753

Figure 4. Transfection of T47D cells with miR-379 or a non-targeting control (NTC) mimic.

Figure 4

(A) Confirmation of elevated miR-379 expression 48 hours following transfection with miR-379 mimic. (B) Cell proliferation following transfection, resulting in a decrease in proliferation (range 7-24%) in the presence of a miR-379 mimic (p<0.05). (C) The effect of miR-379 transfection on Cyclin B1 protein expression was also determined at 48 hours through western blot (C) and immunohistochemistry (D) (i, ii) antibody-free, (iii, iv) Cells transfected with NTC mimic, (v, vi) Cells transfected with miR-379 mimic for 48h, observed significantly reduced Cyclin B1 expression. Magnification (i, iii, v) 10X (50µm scale bar) and (ii, iv, vi) 20X (20µm scale bar).