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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2013 Apr 17;12(7):1276–1287. doi: 10.1158/1535-7163.MCT-12-0841

Figure 2.

Figure 2

Cell cycle arrest in ATC caused by resveratrol treatment. A, Detection of cyclinB1and p21 protein expression by Western blot in HTh7 and 8505C cells treated with three different concentrations of resveratrol (10, 25, 50μM) or vehicle control. Equal loading was confirmed with β-actin. B, Resveratrol caused S phase arrest in both HTh7 and 8505C cells. ATC cells were exposed to different concentrations of resveratrol for 24 hours before they were labeled with BrdU and 7 AAD for flow cytometry analysis. Percentages denote the cells in each cell cycle phase from total single cell population. Results of one representative experiment. Data from three repeated experiments are summarized in bar graph (C). ATC cells with resveratrol treatment were accumulated in S phase while diminished in both G0-G1 and G2-M phases. Data are shown as mean ± SD (n=3, *P<0.05, **P<0.01 vs. control cells without resveratrol treatment).