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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2013 Apr 17;12(7):1276–1287. doi: 10.1158/1535-7163.MCT-12-0841

Figure 6.

Figure 6

Differentiation markers expression in ATC cells was dependent upon Notch1 signaling. A, NICD protein expression levels detected by western blot in 8505C cells, as well as Notch1 mRNA levels quantified by real-time RT-PCR (B). The cells were first exposed to non-specific siRNA (NS siRNA) or Notch1 siRNA and then treated with resveratrol or vehicle control. Notch1 siRNA was effective to block the induction of Notch1 by resveratrol treatment. Equal loading of protein lysate was confirmed with β-actin. C, The quantified mRNA levels of differentiation markers including TTF1, TTF2 and Pax8, as well as the levels of NIS (D)in 8505C cells by real-time RT-PCR. All data were plotted relative to cells treated NS siRNA and vehicle control. All values were expressed as mean± SD (n=3, *P<0.05 and **P<0.01 compared with control).