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. Author manuscript; available in PMC: 2013 Jul 10.
Published in final edited form as: Biochem Cell Biol. 2008 Jun;86(3):227–234. doi: 10.1139/o08-035

Fig. 4.

Fig. 4

Ectopic expression of VHL, but not Flag-VHL induces intercellular junctions and down-regulates integrin expression. (A) VHL-negative 786-0 cells containing pCR3 and 786-0 cells ectopically expressing VHLp18(MEA), Flag-VHLp18(MEA) (FMEA), VHLp24(MPR) or Flag- VHLp24(MPR) (FMPR) were grown on coverslips for 3 days post-confluence. Phase contrast images are shown in the top panels. Bar, 100 µm. Cells were then costained for β-catenin (middle row) and ZO-1 (lower row). Images were captured using a high-resolution AxioCam MRm digital camera on a Zeiss Axiovert 200M microscopy. Bar, 20 µm. (B) Western blot of cell extracts from cell lines shown in panel A. Cells were harvested 3 days post confluence and immunoblotted with antibodies indicated to the left of the blots. β-tubulin was used to confirm equal protein loading.