Skip to main content
. Author manuscript; available in PMC: 2013 Jul 10.
Published in final edited form as: Biochem Cell Biol. 2008 Jun;86(3):227–234. doi: 10.1139/o08-035

Fig. 5.

Fig. 5

Integrins co-immunoprecipitate with VHL but not Flag-VHL. (A) 786-0 cells stably expressing VHLp18(MEA) or VHLp24(MPR) cells were harvested after reaching confluence and cell lysates were immunoprecipitated with rabbit anti-VHLp24(MPR) polyclonal antibody 888. Immunoprecipitates were collected on protein G plus-Agarose beads. (B) VHL-negative 786-0 cells containing pCR3 and 786-0 cells ectopically expressing Flag-VHLp24(MPR) (FMPR) were harvested after reaching confluence and cell lysates were immunoprecipitated with anti-FLAG M2-Agarose. Immunoprecipitates were immunoblotted with antibodies corresponding to the proteins shown to the left of the blots. VHL was detected using mAb 11E12.