Fig 2.

The growth of cancer cells, but not normal cells is sensitive to RMP depletion. The plasmids for RMP overexpression (RMPo), RMP interference (RMPi), or vector alone (Vector) were introduced into various cancer cell lines (A-E) and normal cell lines as indicted (F, G). Untransfected (Unt) parent cells were applied as a control. The growth of cells was determined by MTT assay as described in Material and Methods. Human normal hepatic cell lines HL-7702 (H), L02 (I) and HCC cell lines of HepG2 (J) were transfected with expression plasmids as indicated. (K) HCC cell lines of SMMC-7721 stably depleted of RMP (RMPi), were transiently transfected RMP expression plasmid (RMPi+RMP) or vector alone (RMPi+Vector). The SMMC-7721 cells stably overexpressing RMP (RMPo), cells transfected with vector alone (Vector) and untransfected (Unt) parent cells were applied as controls. The growth of cells was determined by MTT assay everyday starting 24 hours after transfection as described in Material and Methods. (L) The expression of RMP in the transfected SMMC-7721 cells was determined by Western blot analysis as described in Materials and Methods. Results are reported as mean +/-S.D. of three independent experiments.