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. 2013 Jul 5;9(6):637–648. doi: 10.7150/ijbs.6439

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 3 — RMP depletion induces apoptosis in HCC cells, but not in normal hepatic cells. (A) HCC cell lines (SMMC-7721 and HepG2) and normal hepatic cell lines (HL-7702 and L02) were transfected with expression plasmids as indicated and subjected to apoptosis analysis by flow cytometry 72 hours after transfection. (B) The percentage of apoptotic cells were scored and represented in histogram with bar as mean +/-S.D. of three independent experiments. (C) HCC cells SMMC-7721 were transfected with expression plasmids as indicated. 24 hours later the transfected cells were subjected for irradiation at 6 Gy and then cultured for additional times as indicated. Finally cells were harvested and subjected to apoptosis analysis by flow cytometry 48 hours after irradiation. (D) The percentage of apoptotic cells were scored and represented in histogram with bar as mean +/-S.D. of three independent experiments.

Fig 3 — RMP depletion induces apoptosis in HCC cells, but not in normal hepatic cells. (A) HCC cell lines (SMMC-7721 and HepG2) and normal hepatic cell lines (HL-7702 and L02) were transfected with expression plasmids as indicated and subjected to apoptosis analysis by flow cytometry 72 hours after transfection. (B) The percentage of apoptotic cells were scored and represented in histogram with bar as mean +/-S.D. of three independent experiments. (C) HCC cells SMMC-7721 were transfected with expression plasmids as indicated. 24 hours later the transfected cells were subjected for irradiation at 6 Gy and then cultured for additional times as indicated. Finally cells were harvested and subjected to apoptosis analysis by flow cytometry 48 hours after irradiation. (D) The percentage of apoptotic cells were scored and represented in histogram with bar as mean +/-S.D. of three independent experiments.