Figure 1.

Strategy for generating PCCA hypomorph mice. Depiction of the insertion cassette microinjected into FVB mouse fertilized eggs (top). Two separate hypomorph constructs were injected, one with an A138T mutation and another with an A75P mutation. IRES-GFP DNA sequence was included to aid in genotyping founder mice. (bottom) Breeding strategies of Pcca^−/− and Pcca^−/−(A138T) mice. The A138T transcript increases survival well beyond the 36 hours observed in full knockout mice. CAG, cytomegalovirus early enhancer coupled with chicken β-actin promoter; EGFP, enhanced green fluorescent protein; IRES, internal ribosome entry site.