Figure 1.

Experimental strategy and results from proteomic screening. (A) Schematic diagram depicting the region of GBM1 specimen for proteomic screening. (B) Glial fibrillary acidic protein (GFAP or E9PAX3_HUMAN), one of the top-ranked proteins shown in Table 1, was highly expressed in GBM1 vs. normal brain region specimens. The annotated MS/MS spectrum shown illustrates the amino acid sequence assignment of product ions to the top-ranked tryptic peptide, VDFSLAGALNAGFK, which spans amino acid residues 50–63 of GFAP. The insert shows the amino acid sequence coverage of GFAP with tryptic peptides observed in bold (and peptide 50–63 underlined). (C) Abundant level of GFAP and doublecortin (DCX) in independent GBM1 specimens by western blot.