Fig. 10.

Intracellular Ca²⁺ level in normal and genetically modified CRC cells. A, Fluorescent image showing enhanced intracellular Ca²⁺ level in metastatic SW620 cells compared with primary HCT116 and SW480 cells. Statistical significance was calculated using one way analysis of variance method. B, Fluorescent image showing enhanced intracellular Ca²⁺ level in HCT116-CacyBP OE cells compared with HCT116-Control cells and reduced intracellular Ca²⁺ level in SW620-CacyBP KD cells as compared with SW620-Scr Control cells. Green channel indicates Fluo 3AM staining for intracellular calcium. Statistical significance was calculated using two tailed unpaired t test. Quantitation values for fluorescent intensity/area (mm²) were represented as mean ± S.E. (n = 20) based on two independent experiments where ns indicates not significant, * indicates p < 0.05, ** indicates p < 0.001 and *** indicates p < 0.0001.