Fig. 2.

Validation of CacyBP overexpression and knock-down. A, Western blot validation of CacyBP expression level in HCT116-Control, HCT116-CacyBP OE, SW620-Scr Control and SW620-CacyBP KD cells. Actin was used as a loading control. B, Immunofluorescence analysis confirming overexpression of CacyBP in HCT116-CacyBP OE cells compared with HCT116-Control and knock-down of CacyBP in SW620-CacyBP KD cells compared with SW620-Scr Control cells. Red channel indicates CacyBP staining. C, Quantitation for fluorescent intensity for CacyBP expression level. Data represented as mean ± S.E. (n = 25) based on two independent experiments where *** indicates p < 0.0001 using two tailed unpaired t test.