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. 2013 Mar 29;12(7):1865–1880. doi: 10.1074/mcp.M112.023085

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 9. — Validation of iTRAQ results. A, Western-blot studies to validate differential expression pattern of 3 candidate proteins from OE data set: Annexin A2 and nucleolin were down-regulated whereas MARCKS was up-regulated in CacyBP- OE cells compared with the control cells which were in conformity with the iTRAQ results. B, Western blot validation of 5 candidate proteins from KD data set: clathrin, kinesin, calpain and VDAC-1 were found to be down-regulated whereas nucleolin was up-regulated. Actin was used as a loading control in both the cases.