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. 2013 Apr 9;12(7):1926–1938. doi: 10.1074/mcp.M112.024638

Fig. 5.

Fig. 5.

Capillary-morphogenesis of control and VEGF-challenged ECFCs, before and after siRNA treatment. The left column of pictures shows capillary morphogenesis of not stimulated ECFCs. The right column of pictures shows capillary morphogenesis of ECFCs in the presence of VEGF (50 ng/ml). Numbers in each figure indicate the percentage occupancy of optic field by ECFCs capillary projections (±S.D.). Images were collected and analyzed by ImageJ software. Percentage occupancy of control ECFCs treated with RiboFECT and siCONTROL, was taken as 100%. Pictures shown are representative of four different experiments, performed in triplicate. The two-tailed, non-paired Student's t test was performed. * indicates a p value < 0.05. RiboFECT: treatment with transfection reagents alone; siCONTROL: treatment with not-targeting siRNA; siPRDX2: treatment with siRNA smart pool targeting Peroxiredoxin-2; siPRDX6: treatment with siRNA smart pool targeting Peroxiredoxin-6; siCAPNS1: treatment with siRNA smart pool targeting Calpain small subunit 1; siGRP78: treatment with siRNA smart pool targeting 78 kDa-glucose-regulated-protein; siANXA3: treatment with siRNA smart pool targeting Annexin A3.