Fig. 5.

Induction of cyclin B-Luc by Taxotere in hollow fibers. a Bioluminescence images of hollow fibers containing HCT116-cyclin B-Luc cells after vehicle or Taxotere treatment. b–d Nude mice bearing hollow fibers filled with HCT116-cyclin B-Luc cells were either vehicle-treated or treated with Taxotere (i.p., 20 mg/kg). Bioluminescence images (b) were acquired before and at 48 h after treatment, and fold induction is expressed as a ratio of posttreatment versus pretreatment bioluminescence (c) data from five mice with ten fibers; error bars standard error. Cell cycle distribution was determined by flow cytometry with cells retrieved from hollow fibers at 48 h after Taxotere treatment (d).