Table 1. Association Constants and Spectroscopic Parameters for the Binding of NS521 to Various Analytes.
| amine guest | Ka (M-1)a | Isat/I0b (λex = 488 nm) | quantum yield (Φ) |
|---|---|---|---|
| epinephrine | 0 | nd | nd |
| norepinephrine | 78 | 5.4 | 0.0033 |
| dopamine | 112 | 3.0 | nd |
| glutamate | 10 | 7.8 | 0.0095 |
| lysine | 11 | 15.1 | nd |
| glycine | 8 | 11.1 | nd |
| none | 0.0053 |
a
Ka measured by fluorescence spectroscopy, λex = 488 nm, λem = 521 nm. Error in Ka values are ±10% based on triplicate titrations.
b
Isat = fluorescence intensity at saturation taken from the theoretical fit to the binding isotherm. nd = not determined.