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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Microbiol. 2013 Jun 10;89(2):276–287. doi: 10.1111/mmi.12273

Figure 2. YapE adherence to RAW264.7 macrophages.

Figure 2

The ability of YapE orthologs to mediate adherence to eukaryotic cells was determined with RAW264.7 cells. (A) Adherence assays were performed with Y. pestis Δcaf1, ΔpsaA expressing the YapE orthologs. Yp = Y. pestis YapE (pMBL313); Ye = Y. enterocolitica YapE (pMBL311); V = vector control (pMWO-034). (B) Western blot analysis of YapE expression in Y. pestis strains. Black arrowhead indicates processed YapE. Triangle represents addition of increasing concentrations of anhydrous tetracycline in Ye samples to demonstrate that higher levels of expression does not result in cleavage of Ye. (C) Adherence assays with E. coli or (D) E. coli ΔompT expressing the YapE orthologs. YP = Y. pestis YapE (pMBL313); Ye = Y. enterocolitica YapE (pMBL311); V=vector control (pMWO-034). Percent adherence was calculated as the number of cell-associated cfu divided by total cfu from a separate tissue culture well. Three independent biological replicates were performed for each adherence assay and each assay was repeated three times (n=9). Each bar represents the mean percent adherence ± the standard deviation; ** = P≤0.01; *** = P≤0.001; **** = P≤0.0001.