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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Immunol. 2013 May 22;191(1):110–116. doi: 10.4049/jimmunol.1202849

Figure 3.

Figure 3

Cell surface translocation of Ro60 on apoptotic cells is dependent on mY3 RNA. A) Representative northern blot of wild-type fibroblasts subjected to RNA interference with non-targeting control (ctrl), mY1, mY3 or mY1/mY3 small interfering (si) RNA (48 hr, 37°C). B) Northern blot of wild-type fibroblasts co-transfected with siRNA (control, mY1 or mY3) and pBluescriptII KS+ plasmids encoding mY1 or mY3 (48 hr, 37°C). Expression of mY1 (open bars) and mY3 (shaded bars) RNA in wild-type fibroblasts transfected with control, mY1, or mY3 siRNA in the absence (C) or presence (D) of mY1 or mY3 plasmids. E) Flow cytometry analysis of binding of affinity purified anti-Ro60 to early apoptotic fibroblasts transfected with control, mY1, mY3, or mY1/mY3 siRNA relative to untreated fibroblasts. F) Flow cytometry analysis of binding of affinity purified anti-Ro60 to early apoptotic fibroblasts co-transfected with siRNA and plasmid relative to untreated fibroblasts. Values are mean ± SEM for 4 experiments for C-F. *P<0.05, ** P≤0.005.