Figure 4.

Effects of 5′-amino-4-imidazolecarboxamide-ribonucleoside (AICAR) on the EPA- and DHA-induced UCP3 promoter activity in muscle cells. Differentiated C₂C₁₂ muscle cells were transfected with the UCP3 (−1790/+52 bp)/luc reporter gene and pCMV-β galactosidase, were cultured in 1% BSA serum-free media for 40 h with 50 μM of EPA (a) and DHA (b) in the presence of 0.25 mM AICAR. Luciferase activity were calculated in relative light units (RLU) and normalized to β-galactosidase activity. Values are expressed as mean ± SE (n = 3) of three independent experiments. * P < 0.05 and ** P < 0.01 versus control treatment.