Fig. 9.

Transcriptional regulation of ACOT12 in rat hepatocytes. Rat primary hepatocytes were first cultured with 10% FBS and 1 μM of dexamethasone and insulin. Then, 4 h after plating, cells were cultured for the indicated number of days in Williams’ medium E without insulin and dexamethasone in the presence (+) or absence (−) of 10% FBS. Finally, the levels of ACOT12 mRNA (A) and protein (B) were determined by quantitative PCR and immunoblot analysis, respectively. Day 0 means freshly isolated hepatocytes before plating. β-Actin was used as a loading control (Cont). *P < 0.001 as compared with day 0 cells. Rat primary hepatocytes (day 1) cultured without FBS were incubated with or without 100 nM insulin (C, D) or 100 μM clofibrate (E, F) for the time indicated. Finally, the levels of ACOT12 mRNA (C, E) and protein (D, F) were determined. Values are shown as the mean ± SD from three independent culture dishes. *P < 0.05 and **P < 0.001 as compared with control cells incubated with vehicle.