Fig. 1.

SEPS1 gene and protein expression in adipose tissue from lean and obese mice, and during adipogenesis of 3T3-L1 preadipocytes. Epididymal white adipose tissue was isolated from lean and high-fat diet-induced obese mice for 6 weeks (n = 4/group). RNA and tissue homogenates prepared from white adipose tissue were subjected to quantitative RT-PCR and immunoblot analysis, respectively, for analyses of mRNA (A) and protein (B) levels of SEPS1. Adipogenesis was initiated in 2 day postconfluent 3T3-L1 preadipocytes for 6 days by treating with an adipogenic cocktail from day 0 to day 2, insulin-containing medium from day 2 to day 4, and growth medium from day 4 to day 6. Differentiating cells were harvested at days 0, 2, 4, and 6 for analyses of SEPS1 (C), PPARγ (D), and FAS (E) mRNA levels by quantitative RT-PCR. F: Protein levels of SEPS1 were estimated by immunoblot analysis. β-actin was used as a control. Band intensities of SEPS1 normalized by β-actin were quantified using ImageJ 1.45S software. Data represent mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.