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. 2013 Aug;54(8):2069–2082. doi: 10.1194/jlr.M034603

Fig. 2.

Fig. 2.

DEX treatment lowers SEPS1 protein level during the early stage of adipogenesis of 3T3-L1 preadipocytes. Two day postconfluent 3T3-L1 preadipocytes were incubated with the adipogenic cocktail containing DEX, MIX, and insulin (INS) for 1 and 2 days. A: SEPS1 mRNA levels were examined in the early stage of adipogenesis from day 0 to day 2 by quantitative RT-PCR. The signal of β-actin was used for normalization. B: Levels of SEPS1 protein at days 0, 1 and 2 were detected by immunoblot analysis. β-actin was used as a loading control. C: Two day postconfluent 3T3-L1 preadipocytes were stimulated by DEX, MIX, INS, or adipogenic cocktail (DMI) for 48 h. NT, nontreated. Protein samples were subjected to immunoblot analysis for detection of SEPS1 and β-actin levels. Intensity of the protein bands was quantified using ImageJ 1.45S software. Relative SEPS1 intensity was presented after normalization by β-actin band intensity. D: SEPS1 mRNA levels in 3T3-L1 cells stimulated by adipogenic cocktail (DMI) or adipogenic cocktail without DEX (MI) for 2 days were determined by quantitative RT-PCR. Data represent mean ± SEM (n = 3). *P < 0.05; ***P < 0.001.