Fig. 5.

LivKO mice are not insulin resistant. A: The 38 week old LivKO mice versus control male mice showed no changes in plasma concentrations of insulin and glucose in the fed or 14 h fast state (n = 4–5). B: Blood glucose levels in male mice subjected to GTT (at 39 weeks) and ITT (at 40 weeks) (n = 4–5). It appears that LivKO relative control mice tolerated glucose challenge better. C: Acute insulin signaling. The 8–12-week-old LivKO mice and control male littermates were anesthetized and injected with saline (Sal) or human insulin at 0.5 U/kg body weight via the portal vein. Liver, epididymal fat pad (Epi), and quadriceps muscle (Mus) were sequentially collected 5 min after insulin injection and snap-frozen in liquid nitrogen. The insulin-stimulated phosphorylation of Akt threonine 308 (pAkt) and total Akt (T-Akt) in these tissues were detected by immunoblotting using antibodies from Cell Signaling. *P < 0.05.