Fig. 7.

LivKO mice at 42 weeks exhibit no hepatic ER stress. A: Western blots of liver proteins related to ER stress. α-Tubulin was used as a loading control. B: XBP-1 splicing assay by reverse transcriptase PCR using total hepatic RNAs. C: Hepatic mRNA levels of ER stress-responsive genes activating transcription factor 6 (ATF6), CHOP, and BiP measured by qPCR. Cyclophilin was used as an internal invariant control. PDI, protein disulfide isomerase; PERK, protein kinase-like endoplasmic reticulum kinase; p-IRE1α, phosphorylated-IRE1α ERO1Lα, endoplasmic oxidoreductin-1-like α.