Fig. 3.

IDOL-mediated degradation of the LDLR does not require ARH. A: Human fibroblasts (parental) and their engineered derivatives in which WT LDLR or Y828C LDLR were stably introduced and cultured in the absence (−) or presence (+) of 1 μM GW3965 for 24 h. Total cell lysates were immunoblotted as indicated. B: A431 cells were transfected with 20 nM control or ARH siRNA. Subsequently, cells were shifted to sterol-depletion medium for 16 h and then treated for an additional 6 h with 2 μM GW3965. Total cell lysates were immunoblotted as indicated. Immunoblots are representative of three independent experiments.