TABLE 1.

Primers and PCR conditions used to measure placental expression of the inflammatory genes, lipoxygenase enzymes, and reference genes by real time RT-PCR

Gene	Forward/Reverse Primer Sequence	Annealing Temp.(°C)	Amplicon Size (bp)	MgCl2 (mM)
Alox15	F 5′ CACCGGAGACTCCAAGTACG 3′	60	153	3
	R 5′ AGTGGCCCAAGGTATCCTGA 3′
Alox15b	F 5′ CCCTGTTATCAGTGGCTGGA 3′	60	190	3
	R 5′ TCACGGTCTCATGGTCAAGG 3′
Alox5	F 5′ TGGCATCTAGGTGCAGTGTG 3′	60	133	3
	R 5′ CCTCCAGGTTCTTGCGGAAT 3′
Alox5ap	F 5′ GAGAAGCTTCCAGAGGACGG 3′	60	154	3
	R 5′ ATACATCAGCCCAGCGAAGG 3′
Tnfα	F 5′ TACTGAACTTCGGGGTGATTGGTCC 3′	60	295	3
	R 5′ CAGCCTTGTCCCTTGAAGAGAACC 3′
Il6	F 5′ TCCGCAAGAGACTTCCAGCCAGT 3′	60	148	2
	R 5′ AGCCTCCGACTTGTGAAGTGGT 3′
Ptgs1	F 5′ TGCCCTCTGTACCCAAAGAC 3′	60	104	2
	R 5′ CTCCCTTCTCAGCAGCAATC 3′
Ptgs2	F 5′ GAAGGGACACCCTTTCACAT 3′	59	178	4
	R 5′ TGGGGAGACCATGGTAGAAC 3′
Ppia	F 5′ AGCATACAGGTCCTGGCATC 3′	62	127	3
	R 5′ TTCACCTTCCCAAAGACCAC 3′
Sdha	F 5′ TGGGGCGACTCGTGGCTTTC 3′	60	134	2
	R 5′ CCCCGCCTGCACCTACAACC 3′
Ywhaz	F 5′ GACGGAGCTGAGGGACATCTGC 3′	60	75	2
	R 5′ GGCTGCGAAGCATTGGGGATCA 3′