. 2013 Aug;54(8):2282–2290. doi: 10.1194/jlr.D038653

TABLE 1.

Kinetic constants obtained by nonlinear regression analysis

Assay	Substrate	K_m (μM)^a	V_max [nmol/(min·mg)]^a
LPAAT	Alkyne-OLPA	0.53 ± 0.18^b	6.83 ± 4.39^c
LPAAT	PLPA	3.64 ± 0.24^b	2.05 ± 0.21^c
LPCAT	Alkyne-OLPC	2.46 ± 1.62^d	0.51 ± 0.08^e
LPCAT	PLpPC	5.46 ± 2.23^d	0.30 ± 0.07^e
CerS	Alkyne-sphinganine	2.29 ± 2.08	0.71 ± 0.38

Mean values and standard deviations from three independent triplicate determinations. Enzyme preparations, acyl chain donors, and further assay conditions used are given in the text.

P< 0.0001, ^cP = 0.1327, ^dP = 0.1325, ^eP = 0.0267 (these P values refer to the comparison of different substrates in the same enzymatic assay; two-tailed P values from Student's unpaired t-test).