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Specificity of gene silencing. (A and B) Bright field and (A’ and B’) fluorescent field. (A and A’) Co-transfection of the rMC4R-EGFP expression vector with a vector expressing shRNA for MC4R. (BandB’) Co-transfection of the rAgRP-EGFP expression vector with a vector expressing shRNA for MC4R. MC4R-shRNA suppresses rMC4R-EGFP expression but has no effect on rAgRP-EGFP expression.
