Dicer mRNA expression. Images of dark-field autoradiograms showing Dicer mRNA expression in the rat brain. (A and B) Adjacent brain sections at the coronal planes of the hypothalamus were used for in situ hybridization with 35S-labeled sense and antisense (A’ and B’) Dicer cRNA probes. (A and A’) Rostral level and (B and B’) caudal level. ARC, arcuate nucleus; CA1–3, area 1–3 Ammon’s horn; CTX, cortex; DG, dentate gyrus; DMH, dorsomedial hypothalamus; Hb, habenula; PVN, paraventricular nucleus of hypothalamus; TH, thalamus; VMH, ventromedial hypothalamus.