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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Virol Methods. 2013 May 6;192(0):39–43. doi: 10.1016/j.jviromet.2011.11.030

Table 2.

Detection of Mutant HIV Genotypes by Singleplex- versus Multiplex-OLA

HIV-1 Subtype B Codons
HIV-1 Subtype C Codons
103 N (%) 181 N (%) 65 N (%) 184 N (%) 103 N (%) 181 N (%) 106 N (%) 190 N (%)
Total Specimens Tested 49 49 72 72 92 92 90 90
Indeterminate in SPX OLA 3 (6) 2 (4) 4 (6) 5 (7) 5 (5) 8 (9) 0 (0) 5 (6)

Total Compared in MPX vs. SPX 46 47 68 67 87 84 90 85
Mutants detected by SPX 14 (30) 4 (9) 0 (0) 14 (21) 16 (18) 22 (26) 8 (9) 12 (14)
Mutants detected by MPX 14 (30) 3 (6) 1 (1) 14 (21) 14 (16) 23 (27) 11 (12) 13 (15)

Difference in detection (p≤)a 1.00 1.00 1.00 1.00 0.50 1.00 0.25 1.00

Sensitivity of MPX 1.00 0.75 N/Ab 1.00 0.88 0.98 1.00 1.00
Specificity of MPX 1.00 1.00 0.99 1.00 1.00 1.00 0.96 0.99
a

P-values calculated by McNemar’s exact test;

b

Not applicable as no mutants detected by SPX-OLA.