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. 2013 May 31;30(8):1955–1965. doi: 10.1093/molbev/mst102

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 2. — 2A-like sequences and activity assays. 2A-like sequences of non-LTRs (plus the 20 aa downstream of the cleavage site) are shown together with FMDV 2A, for comparison. The 2A region is highlighted by the gray box. Residues conforming to the consensus motif are indicated in bold, those key residues which differ being underlined. Sequences are arranged by their order arising from sequence alignment (supplementary data, Supplementary Material online) (A). Coupled transcription/translation rabbit reticulocyte lysates were programmed with plasmid DNA as indicated (ordered as in A) and protein synthesis de novo monitored by the incorporation of ³⁵S-methionine. Translational recoding or “cleavage” activity was determined by the distribution of radiolabel within either the “uncleaved” form ([GFP-2A-GUS]) or the “cleavage” products ([GFP-2A] plus GUS) (B).

Fig. 2. — 2A-like sequences and activity assays. 2A-like sequences of non-LTRs (plus the 20 aa downstream of the cleavage site) are shown together with FMDV 2A, for comparison. The 2A region is highlighted by the gray box. Residues conforming to the consensus motif are indicated in bold, those key residues which differ being underlined. Sequences are arranged by their order arising from sequence alignment (supplementary data, Supplementary Material online) (A). Coupled transcription/translation rabbit reticulocyte lysates were programmed with plasmid DNA as indicated (ordered as in A) and protein synthesis de novo monitored by the incorporation of ³⁵S-methionine. Translational recoding or “cleavage” activity was determined by the distribution of radiolabel within either the “uncleaved” form ([GFP-2A-GUS]) or the “cleavage” products ([GFP-2A] plus GUS) (B).