Table 1.
Human pluripotent cell lines used in this study
| Cell Line Information | NC Cell Formation | |
|---|---|---|
| iPS-1 | WTC-10 integration-free iPS | Yes |
| iPS-2 | WTB-6 integration-free iPS | Yes |
| iPS-3 | WTC-11 integration-free iPS | Yes |
| iPS-4 | WTC-11 integration-free-H2B-GFP | Yes |
| H9 | Human ES stem cell line | Yes |
The LSB-short NC differentiation method was tested on multiple human pluripotent stem cell lines. All starting pluripotent lines could be differentiated into NC cells as verified by ICC or flow cytometry analysis. Cell lines are referenced as iPS-X for ease of reference throughout the manuscript.