Table 1.
Summary of HCN channel properties, membrane properties and synaptic properties of dorsal and ventral neurons for P18 and P22.
| P18 | P22 | |||||
|---|---|---|---|---|---|---|
| Ventral | Dorsal | P | Ventral | Dorsal | P | |
| HCN CHANNEL PROPERTIES (AT −110.5 mV) | ||||||
| Current [pA] | −3006 ± 165 (15) | −1909 ± 277 (18) | ** | −3303 ± 134 (8) | −2559 ± 238 (9) | * |
| Current density [pA/pF] | −127.7 ± 5.3 (15) | −73.4 ± 9.3 (18) | *** | 122.4 ± 4.9 (6) | 92.7 ± 5.0 (9) | * |
| Half-maximal activation voltage [mV] | −79 ± 1 (15) | −87 ± 2 (18) | ** | −76 ± 3 (8) | −81 ± 2 (9) | n.s. |
| τweighted [ms] | 95.6 ± 7.0 (15) | 191.3 ± 28.1 (18) | ** | 78.0 ± 3.6 (8) | 109.6 ± 18.7 (9) | n.s. |
| MEMBRANE PROPERTIES (AT −100 pA) | ||||||
| Resting potential [mV] | −58.8 ± 0.3 (24) | −59.1 ± 0.6 (35) | n.s. | −60.0 ± 0.7 (10) | −60.1 ± 0.9 (12) | n.s. |
| Input resistance [MΩ] | 10.7 ± 1.8 (24) | 18.9 ± 5.5 (35) | n.s. | 3.7 ± 7.0 (10) | 24.0 ± 6.4 (12) | * |
| Membrane time constant [ms] | 0.69 ± 0.09 (24) | 1.23 ± 0.27 (35) | n.s. | 0.45 ± 0.07 (10) | 1.64 ± 0.45 (12) | * |
| SYNAPTIC PROPERTIES | ||||||
| IPSP half-width [ms] | 3.76 ± 0.25 (13) | 5.63 ± 0.60 (10) | ** | 2.72 ± 0.06 (10) | 4.29 ± 0.51 (13) | * |
| Rise time 10–90% [ms] | 1.10 ± 0.01 (13) | 1.12 ± 0.03 (10) | n.s. | 1.14 ± 0.03 (10) | 1.37 ± 0.07 (13) | * |
| Decay time 90–10% [ms] | 2.51 ± 0.16 (13) | 4.05 ± 0.56 (10) | ** | 1.84 ± 0.08 (10) | 3.08 ± 0.39 (13) | ** |
Values are mean ± SEM. The level of significance between ventral and dorsal was determined by using Student's unpaired t-test except for HCN channel properties of P18, where Scheffe's post-hoc test was employed following a single-factor ANOVA test (
*
P < 0.05,
**
P < 0.01,
***
P < 0.001,
n.s. not significant). The n-values for each group are stated in brackets.