FIGURE 2.

(A) HEK-293 cell lines that express pri-miR-122 or pri-miR-122 p3 were established. The mutant mature miR-122 sequence is shown above. RT-PCR was used to detect the pri-miRNA transcripts and U1 snRNA from each cell line treated with tetracycline or mock-treated. (B) Depiction of capped and polyadenylated RLuc reporter RNAs with or without tandem fragments derived from the CLIC4 3′ UTR. “X” indicates seed C to G mutation at p3 in predicted miR-122 seed-binding sites. (C) Reporter RNAs shown in B were cotransfected along with FLuc RNA into HEK-293 cells either mock-treated or tetracycline-treated for 48 h prior to transfection. For each RNA, the mock-treated condition was set to 1.0 for comparison to the tetracycline-treated condition. Error bars indicate SD values.