FIGURE 4.

Cell-free translation reactions using WT and p3 HCV16LUC reporter RNAs were conducted using lysates derived from mock-treated (A) or tetracycline-treated (B) HEK-293 cells that harbor an inducible pri-miR-122 transgene. At the indicated time points, aliquots of reaction mixtures were removed for measurements of RLuc activity. The data shown are representative of multiple independent experiments. Data from A and B are shown together in panel C. A separate experiment performed in triplicate with a single 120-min time point is shown in panel D. For each lysate (mock or tet), the p3 reporter RNA was normalized to 1.0 for comparison to the WT reporter RNA. A similar experiment was performed with the same reporter RNAs in lysates from cells harboring the p3 pri-miR-122 mutant transgene (E). In panel E, the WT reporter RNA was normalized to 1.0 for each lysate (mock or tet) for comparison to the p3 mutant. All error bars indicate SD values.