FIGURE 5.

Cell-free translation reactions were assembled with either WT or p3 mutant HCV16LUC reporter RNAs using lysate from miR-122-expressing cells as in Figure 4B, except that transcripts were internally radiolabeled. At the indicated time points, reaction aliquots were removed for RNA extraction and analysis by denaturing PAGE (A). Levels of rRNA in each corresponding sample were separately evaluated by agarose gel electrophoresis. Phosphorimager analysis of radiolabeled RNA signals is shown in panel B. Levels of RNA before incubation of reactions (time point 0) were calibrated to 100%.