Table 4.
Summary of antibodies used in this study
Target | Species | Clone | Source | Dilution WB | Dilution IFA |
---|---|---|---|---|---|
ATF6α | Mouse | 70B1413.1 | Imgenex | 1:1000 | N/A |
ATF6β | Goat | N‐17 | Santa Cruz | N/A | 1:100 with tyramide amplification |
IRE1α | Rabbit | 14C10 | Cell Signaling | 1:1000 | N/A |
peIF2α | Rabbit | PS52 | Invitrogen | 1:1000 | 1:1000 with tyramide amplification |
eIF2α | Rabbit | N/A | Cell Signaling | 1:1000 | N/A |
ATF4 | Rabbit | C20 | Santa Cruz | 1:1000 | 1:600 with tyramide amplification |
CHOP | Mouse | 9C8 | Thermo Fisher | 1:500 | N/A |
HSP70 | Rabbit | D69 | Cell Signaling | 1:1000 | N/A |
WB, western immunoblotting; IFA, immunofluorescent analysis; N/A, not available.