Figure 1.

Regulation of FGFR3 expression by hypoxia. (A) Expression of FGFR3 at the protein level in the bladder cancer cell lines RT4, RT112, T24 and 97-7. Cells were cultured in normoxia (N) or 0.1% O₂ (H) for 24 h and whole cell lysates were probed for FGFR3, HIF-α (RT4 and 97-7: HIF-1α; RT112 and T24: HIF-2α) and ACTB. Quantification of representative western blots are shown. (B–D) Expression of FGFR3 mRNA in (B) RT4, (C) RT112 and (D) 97-7 cells after exposure to normoxia (white bars) or 0.1% O₂ (black bars) for the indicated time. (A) is representative of at least three independent experiments, (B–D) are mean and s.e.m. of three independent experiments. *P<0.05, **P<0.01, ***P<0.001.